Status of Arbuscular Mycorrhizal Fungi Research in Pakistan: A Comprehensive Review of Diversity, Distribution, and Agricultural Applications
DOI:
https://doi.org/10.65761/jbs.2026.36Keywords:
Arbuscular Mycorrhizal Fungi in Pakistan, Mycorrhizal Diversity and Distribution, Agricultural Mycorrhizology, Sustainable Soil Health, Biofertilizers and Crop YieldAbstract
Background: Around 80% of land vascular plants are obligate symbiotic to Arbuscular Mycorrhizal Fungi (AMF). They are very important to the acquisition of nutrients and the functioning of ecosystems. The role of AMF in stress tolerance is also important.
Methods: Between 1976 and 2020, mycorrhizal studies (80) conducted in Pakistan were reviewed. Studies from Khyber Pakhtunkhwa province were reviewed in detail (30 studies). Various plants were the focus of these studies, including those used for medicine, for agriculture, ornamental plants and even plants like bryophytes and pteridophytes. The studies have been compiled and reviewed for AMF and the plant-AMF symbiosis for the different studies.
Results: The results reveal Glomus was the most common AMF genus in Pakistan. There were Vesicular-arbuscular mycorrhizal (VAM) infection rate results of 0-100%, and most of the plants surveyed had a high degree of infection, greater than or equal to 95%. AMF inoculated plants had a significantly higher uptake of nutrients and had a higher productivity rate in soils that were deficient in phosphorus.
Conclusion: The findings show that AMF that are found in nature have the potential to act as biofertilizers for agriculture in Pakistan and advocate for the veracity of further field tests. There is also a need for the molecular characterization of the native AMF.
References
1. Chandra S, Kehri HK. Biotechnology of VA mycorrhiza: Indian scenario. New Delhi: New India Publishing Agency; 2006.
2. Gorsi M. Studies on mycorrhizal association in some medicinal plants of Azad Jammu and Kashmir. Asian J Plant Sci. 2002;1(4):383-7. doi: 10.3923/ajps.2002.383.387
3. Lebreton A, Tang N, Kuo A, LaButti K, Andreopoulos W, Drula E, et al. Comparative genomics reveals a dynamic genome evolution in the ectomycorrhizal milk-cap (Lactarius) mushrooms. New Phytol. 2022;235(1):306-19. doi: 10.1111/nph.18143
4. Fazal Z, Sher K, Hazrat A. Effects of VAM (vesicular arbuscular mycorrhizae) on growth performance of Mentha longifolia L. at various levels of rock phosphate amendments. FUUAST J Biol. 2018;8(2):311-8.
5. Iqbal U, Gul MF, Naseem Z, Jabeen H, Hameed M, Naz N, et al. Thal desert flora: diversity, abundance, phytochemistry, therapeutic potential, and conservation status. Genet Resour Crop Evol. 2026;73(2):102. doi: 10.1007/s10722-025-02715-2
6. Siddiqui ZA, Pichtel J. Mycorrhizae: an overview. In: Siddiqui ZA, Akhtar MS, Futai K, editors. Mycorrhizae: sustainable agriculture and forestry. Dordrecht: Springer; 2008. p. 1-35. doi: 10.1007/978-1-4020-8770-7_1
7. Tabassum Yaseen TY, Muhammad Ibrar MI, Barkatullah B. VAM infection and VAMF spores in Withania somnifera (L.) Dunal and Withania coagulans (Stocks.) at fruiting stage. Int J Biosci. 2011;1(6):1-8.
8. Brundrett MC. Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant Soil. 2009;320(1):37-77. doi: 10.1007/s11104-008-9877-9
9. Sanders IR. Mycorrhizal symbiosis. By SE Smith and DJ Read. 25 × 17 cm. Pp. ix+605 with 172 text-figures and black-and-white photographs and 4 colour plates. London: Academic Press, 1997. Price h/b: £65.00, ISBN 0 12 652840 3. New Phytol. 1997;137(3):563-8. doi: 10.1046/j.1469-8137.1997.00851-2.x
10. Sarah S, Burni T, Shuaib M, Alzahrani Y, Alsamadany H, Jan F, et al. Symbiotic response of three tropical maize varieties to eco-friendly arbuscular mycorrhizal fungal inoculation in marginal soil. Biocell. 2019;43(5-1):245-52.
11. Jan B, Sharif M, Khan F, Bakht J. Effect of arbuscular mycorrhizal fungal inoculation with compost on yield and P uptake of wheat in alkaline calcareous soil. Am J Plant Sci. 2014;5(13):1995-2001. doi: 10.4236/ajps.2014.513213
12. Singh S, Kapoor K. Inoculation with phosphate-solubilizing microorganisms and a vesicular-arbuscular mycorrhizal fungus improves dry matter yield and nutrient uptake by wheat grown in a sandy soil. Biol Fertil Soils. 1999;28(2):139-44. doi: 10.1007/s003740050475
13. Sarah S, Burni T. Symbiotic response of three tropical sorghum varieties to arbuscular mycorrhizal fungal inoculation in marginal soil. Pak J Bot. 2013;45(S1):401-6.
14. Burni T, Hussain F. Diversity in arbuscular mycorrhizal morphology in some medicinal plants of family Lamiaceae. Pak J Bot. 2011;43(3):1789-92.
15. Reza DTA, Sohrab M, Hossein Ali A, Masoomeh S. Role of rhizospheric microorganisms in mitigating the adverse effect of salinity stress in Plantago ovata growth, biochemical and photosynthetic traits. Arch Agron Soil Sci. 2021;67(8):1060-74. doi: 10.1080/03650340.2020.1775816
16. Verma M, Langer A. Studies on AM associations in Marchantia nepalensis L. et L. Int J Pharm Biol Sci. 2014;9(2):26-9.
17. Baas R, Van Dijk C, Troelstra S. Effects of rhizosphere soil, vesicular-arbuscular mycorrhizal fungi and phosphate on Plantago major L. ssp. pleiosperma Pilger. Plant Soil. 1989;113(1):59-67.
18. Sujatha V. Standardization of nursery techniques in Melia azedarach L. [master's thesis]. Coimbatore: Tamil Nadu Agricultural University; 2014.
19. Henkel TW, Kelloff CL, Alexander SN, Funk VA. Smithsonian plant collections, Guyana: 1992-2014. Washington (DC): Smithsonian Institution Scholarly Press; 2016. doi: 10.5479/si.1938-2812.104
20. Sharif M, Moawad A. Arbuscular mycorrhizal incidence and infectivity of crops in Northwest Frontier Province of Pakistan. World J Agric Sci. 2006;2(2):123-32.
21. Redecker D, Kodner R, Graham LE. Glomalean fungi from the Ordovician. Science. 2000;289(5486):1920-1. doi: 10.1126/science.289.5486.1920
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Naji Ullah, Muhammad Junaid Khan, Asma Bibi, Irfan Khan, Salman Khan

This work is licensed under a Creative Commons Attribution 4.0 International License.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.